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| Giffard Mena, I; Lorin Nebel, C; Charmantier, G; Castille, R; Boulo, Viviane. |
| Sea-bass (Dicentrarchus labrax) grow under different salinity regimes, from the open sea to lagoons and even rivers, but some mortality has been recorded in juvenile stages when exposed to low salinity water. Changes in water permeability of different osmoregulatory tissues could be the cause of reduction in blood osmotic pressure and death in some fish in fresh water (FW). In order to explore this condition, we have studied the changes of aquaporins (AQP1 and AQP3), alpha 1 and alpha 4 Na+/K(+-)ATPase transcript levels in the digestive tract, kidney and gills after a long-term exposure of juvenile sea-bass to sea water (SW) and FW fish able to survive in SW and FW are called SW-adapted fish (SWS), FW successfully-adapted fish (FWS) respectively, while... |
| Tipo: Text |
Palavras-chave: Salinity adaptation; Juvenile sea bass; Differential mortality; Alpha 4 Na+/K+ ATPase; Alpha l Na+/K+ ATPase; AQP3; AQP1. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4656.pdf |
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| Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R. |
| The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of... |
| Tipo: Text |
Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf |
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| Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G. |
| This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after... |
| Tipo: Text |
Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf |
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